Cultivation Equipment

Cultivation Equipment

Cultivating mushrooms at home, whether you're a beginner or a seasoned mycologist, requires a collection of crucial equipment and supplies. These tools ensure that your cultivation process is efficient, and the likelihood of contamination is significantly reduced. Here, we outline the essential items you'll need to successfully cultivate mushrooms at home, grouped by their purpose and use.

Mushroom Grow Kits

If you're a beginner, mushroom grow kits serve as an excellent introduction to the world of mycology. They conveniently bypass the more complex initial stages of mushroom cultivation, offering a pre-sterilized substrate and sometimes even fully colonized mycelium, allowing you to jump right into the exciting parts of the process like fruiting and harvesting.

Oyster Mushroom Grow Kit

Experience the magic of mushroom cultivation with the Organic Oyster Mushroom Grow Kit. Just 10 days to gourmet, home-grown delights, all made in the USA.

Pink Oyster Mushroom Grow Kit

Experience the magic of mushroom cultivation with the Organic Pink Oyster Mushroom Grow Kit. Just 10 days to gourmet, home-grown delights, all made in the USA.

Lion's Mane Mushroom Grow Kit

Want to grow Lion's Mane at home? This USDA Certified Organic kit from North Spore is my go-to. Easy to use and beginner-friendly, it's a must-have for anyone interested in mycology.

Where Can You Buy Quality Spores and Cultures?

Obtaining high-quality spores or cultures is a foundational step in mushroom cultivation. While many online sources claim to offer the best, it's essential to do your homework. Checking reviews and seeking recommendations can go a long way in ensuring you get a quality product.

Here are some of the top-notch cultivators I personally vouch for:

  • Spore N' Sprout: It's my first stop when I'm hunting for new cultures. They offer clean, affordable options, and quick delivery.
  • Fresh From the Farm Fungi: Gary is an excellent mycologist. Every culture I've snagged from him has been stellar.
  • North Spore: A leading brand in the world of mycology. I've purchased Wine Cap spawn from them for outdoor cultivation.

Sterilization & Sanitation

Sterilization and sanitation form the cornerstone of successful mushroom cultivation. A pressure cooker or an autoclave ensures that your equipment and substrates are sterile, setting the stage for healthy mushroom growth. Additionally, disinfectants like isopropyl alcohol and bleach are indispensable for cleaning surfaces and tools, helping you further minimize contamination risk.

Other sterilization aids include alcohol lamps for metal tools, and Still Air Boxes (SAB) or Laminar Flow Hoods to create sterile environments during transfers and inoculations. Personal protective equipment like gloves and face masks also play a crucial role in maintaining hygiene and preventing contamination. For those interested, we have a comprehensive guides on:

23-Quart Pressure Cooker

Sterilization is key in mycology, and the Presto 23-Quart Pressure Cooker has never let me down. I use and wholeheartedly recommend it!

All American 25qt Pressure Cooker

On my wishlist for its versatility and quality, the All American 1930 Pressure Cooker/Canner promises easy and secure sterilization for all your mycology needs. Made in the USA and suitable for multiple types of stoves.

Kollea Torch Lighter

Ensure the Sterilization of your tools with the Kollea Torch Lighter. Featuring a windproof triple jet flame, safety lock, and a handy punch – your ideal companion for mycology. (Butane not included. Silver finish.)

Alcohol Burner Lamp

Keep your mycology tools sterilized and ready with this Classic Style Alcohol Burner Lamp. Comes with 5 free replacement wicks and a funnel, making it a hassle-free addition to your lab equipment

Nitrile Gloves - Black

With sizes from XS to XL and a 100-count box, these Disposable Nitrile Exam Gloves are latex-free and powder-free, ensuring a snug fit and ample supply for multiple mycology sessions.

Stainless Steel Wire Rack

Working in a sterile environment? The Stainless Steel Wire Rack ensures your project remains elevated in a still air box or flowhood, minimizing exposure to falling airborne debris.

Stainless Steel Surgical Tray - 3 Pack

I regularly use and recommend these Stainless Steel Surgical Trays for my mycology work. Their versatility provides a clean and organized surface for an array of tasks.

Clear Latching Storage Box - 106 Quart

Looking to create an efficient Still Air Box (SAB)? This 106-Quart Clear Latching Storage Box will do the job. It's the perfect size and clear.

Portable UV Sanitizer Lamp

UVC light can assist in disinfecting your projects, enhancing the likelihood of successful outcomes. UVC light in the 200-280NM wavelength is effective in eliminating bacteria and viruses.

Tools & Measuring

Success in mushroom cultivation is significantly bolstered by using the right tools and measuring equipment, which provide a foundation of accuracy and precision. Digital scales are essential for weighing both ingredients during the preparation phase and the mushrooms after harvest. Erlenmeyer flasks, Graduated Cylinders, and funnels add another layer of exactitude to your measurements, ensuring that your substrate and nutrient solutions are perfectly balanced. A Thermo Gun offers the added advantage of real-time temperature monitoring, making it easier to maintain the optimal environment for mycelial growth.

For tasks that require a delicate touch, such as transferring mycelium, specialized tools come into play. A scalpel is indispensable for this, allowing you to move mycelium from Petri dishes to grain spawn with surgical precision. Sharp knives or scissors are another must-have, crucial for cleanly cutting agar and harvesting mature mushrooms. These items are usually part of a standard dissection kit, offering a convenient package of essential tools for mushroom cultivation. All told, a well-stocked toolkit is a cornerstone of efficient and successful mushroom cultivation.

AMIR Digital Scale (0.05g-500g)

Precision is key with the AMIR Digital Scale. Ideal for measuring small ingredients ranging from 0.05g to 500g. Stainless steel, with two trays for convenience.

Tekcity Digital Scale w/ Bowl

Perfect for the dedicated grower: the Tekcity Digital Scale, complete with bowl. Ideal for measuring substrate, ingredients, and post-harvest mushrooms.

Erlenmeyer Flask x2

My go-to container for LMEA, Erlenmeyer Flasks are perfect! Their ventilated screw-on cap ensures safe pressure cooking while preventing contamination—get your citizen science on!

Graduated Cylinder Set

This Borosilicate Graduated Cylinder Set comes in three sizes—50mL, 100mL, and 250mL—providing versatility for measuring various volumes of liquids in your mycology experiments. The durable borosilicate glass ensures accuracy and longevity.

Measuring Cup 2-Quart - Clear

Measure large amounts of grain, substrate, or liquids with precision using this durable 2-Quart Polycarbonate Measuring Cup, a staple for any mycology project.

Dissection Kit

A comprehensive 37-piece Dissection Kit that's a must-have for any mycologist. Its stainless steel instruments serve multiple uses, from specimen preparation to intricate studies.

Infrared Thermometer

Monitoring temperature is crucial in mycology, and this Infrared Thermometer makes it a breeze. I personally use and recommend it for accurate, non-contact measurements.

Surgical Grade Blades #11 10pcs

Ideal for intricate mycology procedures, these Surgical Grade #11 Blades paired with a #3 Scalpel Handle offer precision you can rely on.

Conversion Chart Magnet

Never miss a measurement again with this handy Conversion Chart Magnet. It offers both imperial and metric conversions for weight, liquid, and temperature, making it incredibly useful for mycology calculations.

Hole Saw Set - 13Pcs Saw Blades

If you're into DIY projects like creating airflow for monotubs or a Still Air Box (SAB), this 13-piece Hole Saw Set from HYCHIKA is indispensable. I use and recommend it for its versatility and quality.

Agar Supplies

Agar serves as a nutritive base where spores can germinate and mycelium can grow. This often takes place in sterile Petri dishes or ketchup cups (K-Cups) for the budget-conscious. The quality of agar and its ingredients can significantly affect the speed and vigor of your mycelium growth.

Ingredients like Activated Charcoal Powder for Black Agar, Agar-Agar Powder for consistency, and Light Malt Extract for nutritional value are some of the key components you can use. Tools like Centrifuge Tubes, Inoculation Loops, and Parafilm are equally important for successful agar work. We even offer a guide on how to make your own Light Malt Extract Agar (LMEA) at home.

Agar-Agar Powered

When it comes to achieving the perfect LMEA consistency, NOW Foods Agar Powder is my go-to choice. I've tested and trust its reliability for all my mushroom cultivation needs.

Light Malt Extract

I've seen my mycelium thrive with Briess Golden Light Dry Malt Extract in my LMEA preparations. Trust me, your mushroom cultures will thank you for it!

Activated Charcoal Powder

I use Activated Charcoal Powder to make Black Agar, a twist on the classic LMEA mix. In my experience, it's great for achieving stronger and faster fungal growth.

Petri Dishes 50 Pack 90mm

These Petri Dishes are perfect for mycology agar plates, and the sterile condition ensures that your cultures stay uncontaminated.

K-Cups for Agar

For mycology, I find these 2 oz. Small Plastic Containers excellent for agar cultures. They're budget-friendly and a good single-use alternative to traditional petri dishes.

Parafilm - All Purpose Laboratory Film

For those serious about mycology, Parafilm M Laboratory Film is a game-changer. It not only shields your cultures from moisture but also allows for essential gas permeability, making it indispensable for sealing petri dishes effectively.

Inoculation Loop

Inoculation loop with 50pcs 10uL Nichrome Needle Tips is your go-to tool for efficient and clean spore transfers onto agar plates. An addition to any mycologist's toolkit.

Centrifuge Tubes

Create reliable culture slants with these 50ml Plastic Centrifuge Tubes. The graduated marks help with accurate measurements and the blue screw caps ensure sterility, making them a top pick for any mycology enthusiast.

Liquid Culture Supplies

Liquid cultures serve as a medium for growing large amounts of mycelium in a liquid solution, making the inoculation process faster and more efficient. Sterile disposable injection needles, magnetic stirrers, and syringes are some of the crucial supplies you'll need.

Syringe - 20 Pack

This 20-pack of syringes is ideal for preparing your own spore syringes and liquid cultures. Easy to use and sterilize, they're a must-have for any mycology enthusiast.

Injection Needle - Sterile Disposable

Stock up for all your mycology needs with this 100-pack of 18Ga 1-inch Sterile Disposable Injection Needles. With caps included, they're great for making spore syringes and liquid cultures.

Magnetic Stirrer

I highly recommend this Magnetic Stirrer for all your mycology needs. Whether it's mixing agar or keeping liquid cultures homogenous, this device is a game-changer.

Needle Tip Cap - 150 PCS

These Lock Dispensing Needle Tip Caps are essential for capping your spore syringes or liquid cultures. Secure and leak-proof, they'll keep your solutions sterile until you're ready to use them.

Jars Tek & Spawn Supplies

When it comes to the production of mushroom spawn, jars and grain bags serve as critical mediums for mycelial expansion post-agar stage. I personally use glass jars for their eco-friendly and reusable nature. However, sterilized grain bags are a convenient alternative, as they can be disposed of after use, which can minimize the risk of contamination in subsequent cultivation cycles.

Below, you'll find the products I personally use for modifying jar lids to create optimal conditions for mushroom cultivation. Specialized lids with built-in gas exchange and injection ports allow for necessary air circulation while maintaining a sterile environment for mycelial growth. These products make it easy to inoculate your jars with a spore syringe or liquid culture, without exposing the inner contents to contaminants. Investing in these jar lid modifications can significantly streamline your cultivation process, making it more efficient and far less prone to contamination.

Filter Patch Stickers -20mm x64

Revolutionize your Jar Tek process with these 64Pcs 20mm Filter Discs. Designed for effective air exchange, their strong adhesive feature is an absolute game changer.

High Temperature J-B Weld

Trust in the durability and high-temperature resistance of J-B Weld RTV Silicone when making your own injection ports.

Metal Hole Puncher

Get your lids ready for mycology projects with this Heavy Duty Hole Punch Kit. Ideal for creating air exchange and injection-port holes in metal mason jar lids, it ensures a clean, smooth finish every time.

Plastic Mason Jar Lids

These Mason Jar Lids are a durable and reliable alternative to metal lids. I prefer using them for my grain spawn and liquid cultures, as they provide a secure, leak-proof seal.

Regular Mouth Pint Jars - Set of 12

A set of 12 Regular Mouth Pint Jars that I highly recommend for storing grain spawn or liquid cultures. The size is just right for hobbyists and serious mycologists alike.

Self Healing Injection Ports - x100 13mm

Equip your jar lids with these 13mm Self Healing Injection Ports for fuss-free inoculations. They offer a sterile barrier that heals itself after each injection, perfect for any mycology project.

Bulk Substrate Materials

Once the grain spawn is fully colonized, the next step is to mix it with a bulk substrate like straw, wood chips, or compost. This provides the mycelium with the nutrients it needs during its growth phase. Spawn bags or containers can be used for this mixing process.

Different mushrooms prefer different types of substrates. Understanding the specific needs of the mushroom you're growing can dramatically improve your yield.

EZ Straw

EZ STRAW offers a clean, sustainable option for your mycology substrate needs. Its natural fibers create a conducive environment for mycelium growth and moisture retention.

Impulse Sealer 12 Inch

Make your mycology practice more efficient with this 12-inch Impulse Sealer. It's perfect for securely sealing myco bags, ensuring a sterile environment for mushroom growth.

Masters Mix 10 lbs

Fast Fruiting Masters Mix from MushroomMediaOnline is a blend of 50% Oak and 50% Soy Hull Pellets, making it a go-to substrate for optimized mushroom yields. If you're aiming for quick and prolific fruiting Masters Mix is an excellent choice.

North Spore Boomr Bag (5 lbs)

Take your mushroom cultivation to the next level with North Spore's 'Boomr Bag,' a 5 lb bag of sterile, premium horse manure blend substrate. Made in Maine by expert mycologists, it's formulated for maximum yield and larger flushes.

Fruiting and Harvesting

The final stage, fruiting and harvesting, demands close attention to environmental factors like humidity and temperature. Tools like spray bottles, growth chambers, and humidity and temperature monitors become crucial here.

While natural light can often suffice for fruiting, specialized grow lights can offer more controlled light exposure. The investment in these supplies will definitely pay off when you see your first flush of healthy, mature mushrooms.

2-in-1 Grow Tent - 48x36x72

The VIVOSUN D436 2-in-1 4x3 Grow Tent is a game-changer for indoor mushroom cultivation. With its high-reflective Mylar and multi-chamber design, you can optimize different stages of growth in the same space.

Amber Glass Spray Bottles 16oz - 2 Pack

These Amber Glass Spray Bottles, each holding 16 ounces, are perfect for mycology enthusiasts. Use them for storing isopropyl alcohol for sterilization or water for maintaining optimal humidity conditions.

Crate Pan - Large

The Large Crate Pan is a smart addition to your Martha tent setup, providing a solid foundation and an easy way to manage water runoff, making your mushroom growing experience hassle-free

Fine Mist Spray Bottle

This Fine Mist Spray Bottle delivers a consistent mist, perfect for maintaining the right humidity levels in your grow area. An indispensable tool for anyone serious about mushroom cultivation.

VIVOSUN Fogger 2.5L

The VIVOSUN Fogger is a go-to for consistent humidity control in my mycology projects. This device efficiently generates fog to maintain optimal growing conditions.

Galanz Retro Mini Fridge + Freezer

The Galanz Retro Mini Fridge + Freezer offers a stylish and compact solution for storing cultures or keeping your harvested mushrooms fresh. Its dual functionality makes it a versatile addition to any mycologist's space.

Humidity and Temperature Monitor

Keep track of your growing conditions with this Humidity Gauge with Temperature Monitor. Essential for maintaining the perfect climate for mycology projects.

Inline Duct Ventilation Fan - 4 Inch

This 4-inch Inline Duct Ventilation Fan is a must-have for controlling airflow in your grow tent. Efficient and quiet, it ensures optimal growing conditions by circulating fresh air and expelling excess humidity.

Mini Bluetooth Humidity Sensor

The Govee Hygrometer Thermometer offers wireless convenience and smart alerts, making it a must-have for anyone serious about mushroom cultivation. Monitor humidity levels over time and receive alerts—all via an easy-to-use app.

Temperature and Humidity Controller Outlet Plug

The Temperature and Humidity Controller Outlet is an essential tool for anyone using a grow tent for mushroom cultivation. It allows for precise control of your microclimate, ensuring optimal mushroom growth conditions.

Mini Greenhouse

The 4-Tier Mini Greenhouse is perfect for DIY Martha tents, offering a versatile solution for those looking to optimize their mushroom-growing conditions.


Proper storage is key to preserving the viability of your mushroom cultures and harvested fruit bodies. Refrigerators and dehydrators can be invaluable, while sealable bags and airtight containers with silica packets can preserve the quality of your harvest for a long time.

For cultures, spore prints, and syringes, sterile storage conditions are essential to prevent contamination and prolong their shelf life. Investing in quality storage solutions can save you both time and resources in the long run.

Presto Dehydrator

Keep your harvested mushrooms fresh for longer with the Presto Dehydrator. A must-have for mycologists, it's ideal for long-term storage without sacrificing quality.

Silica Gel Packets

Keep your mushrooms in tip-top condition with Dry & Dry Silica Gel Packets. Designed for long-term storage, these packets are food-safe and effectively reduce moisture, keeping your mushrooms fresh for extended periods.

Cultivation Books

Cultivation books serve as a rich source of information and expertise, guiding you through the complexities of mushroom growing. Whether you're a beginner or a seasoned cultivator, books can offer new insights and techniques that can improve your yield and reduce the risks of contamination.

From basic cultivation methods to advanced mycological studies, a well-rounded library can be your most trusted companion in your mycological journey. Investing in good quality, comprehensive books can provide value that far outweighs their cost.

Growing Gourmet and Medicinal Mushrooms

Whether a novice or a seasoned mycologist, this book unveils the secrets of growing and enjoying a variety of mushrooms to their fullest. by Paul Stamets

Radical Mycology

Fungi, the unsung heroes of our world. Dive deep into their roles, benefits, and the profound effects on life and human culture.

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